random primer

The comparison between PCR and random primer labeled method

PCR和随机引物标记探针的方法比较

Random primer extension radiolabeled DNA probe and its application

随机引物法标记放射同位素DNA探针及其应用

The particular band pattern of dark and light was observed by using random primer PRINS .

结果表明，随机引物延伸在染色体上呈现明暗相间的带纹样特征。

Methods : PCR with an anchoring primer in IS 1541 and random primer ;

方法：用一条锚定的IS1541内部锚定引物和一条随机引物进行PCR扩增；

Detection of Unknown Virus from 2 Case of Aseptic Meningitis Epidemic Using Random Primer PCR Assay

随机引物聚合酶链反应法鉴定2例无菌性脑炎的未知病毒

RAPD molecular markers with the same random primer showed obvious difference in specific fragments amplified between them .

RAPD分子标记结果表明，二者的扩增条带明显不同。

An Extended Random Primer Amplified DNA ( ERPAD ) Marker Linked to a Dominant Male Sterile

甘蓝显性雄性不育基因的延长随机引物扩增DNA（ERPAD）标记

Results and conclusion : Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system .

结果与结论：Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果，且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。

Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes .

分别应用随机引物和SOX基因兼并引物在人类染色体上进行了原位延伸标记。

Using DNA probe method and random primer method , the full DNA sequence of Tm-GRP is screened from the cDNA library of Tenebrio molitor .

利用DNA探针法和随机引物法从大黄粉虫的cDNA文库中筛选TmGRP的全部DNA序列。

However , the essential content of law has its own form which is the inner structural character . coli Klenow fragment random primer DNA labeling system served as control .

以大肠杆菌Klenow片断随机引物延伸标记法作为对照。

Result : 52 pieces of RAPD random primer were selected from all , four pieces of random primers could reflect the difference of andrographitis in different place of production .

结果：共筛选了52条RAPD随机引物，有4条随机引物可综合反映不同产地穿心莲的差异。

The result showed that the concentrations of Taq polymerase , random primer , Mg 2 + and the purity of template DNA played an important role in RAPD analysis .

实验结果表明，Taq酶、随机引物和Mg2＋浓度及模板DNA的纯度对RAPD扩增影响较大。

3 ' Extended random primer amplified DNA ( 3 ' - ERPAD ) markers of Loropetalum chinense var. rubrum

红檵木的3′端延长随机引物扩增DNA（3′-ERPAD）标记

Conclusion : The real time RT-PCR method using 8-mer random primer was much better for investigating mRNA levels of flagellin genes of S. enterica serovar Typhi .

结论：成功构建用8核苷酸随机引物进行逆转录同时测定伤寒杆菌鞭毛抗原z66和d/j基因表达的荧光实时定量RT-PCR方法。

It satisfies the detection of polygenic locus by the amplification of genome . With the use of random primer , PEP may keep a good balance in amplification procedure .

结论PEP是一种建立在特殊反应条件下的微量全基因组扩增技术，其对模板的放大能够充分满足多基因位点同时检测的需要，随机引物的使用使其扩增具有良好的平衡性。

Second , through Parent C and parent F , 105 random primer were selected , and 87 primer acquired amplified products , 592 bands were gotten in all , 19 bands were polymorphism .

通过亲本C、F对105条随机引物进行筛选，共有87个引物获得了扩增产物，出现的谱带总数为592条。

The whole HBV DNA sequences were labelled by random primer incorporation of Digoxigenin - labelled dUTP , and compared with ~ ( 32 ) P-labelled probe by nick translation .

按随机引物法以地高辛标记HBVdna探针，与按缺口翻译法以~（32）P标记的探针进行了比较。

Degenerate Primers Polymerase Chain Reaction Clone and Identification of the Acetylcholinesterase Gene Fragment from the Mosquito , Aedes albopictus ; Detection of Unknown Virus from 2 Case of Aseptic Meningitis Epidemic Using Random Primer PCR Assay

白纹伊蚊乙酰胆碱酯酶基因片段简并引物PCR、克隆及鉴定随机引物聚合酶链反应法鉴定2例无菌性脑炎的未知病毒

Results ASON group reduced the number of cells ( P < 0 01 ), lengthened doubling time ( P < 0 005 ), and elevated the inhibiting rate ( P < 0 01 ) compared with random primer control and empty control .

结果：ASON实验组与随机引物对照组比较能明显减少细胞增殖数目（P<0.01）、增加抑制率（P<0.01）及延长倍增时间（P<0.005）；

RESULTS One kind of optimum random primer was found in every of 8 sorts of bacteria . The differences were observed among the concentrations of optimum reaction system ( key reaction components ) and the greatest difference between the template concentration was up to 100 times .

结果8种细菌各有1条最佳随机引物，相应的优化反应体系（关键反应成分）浓度差别较大，其中模板浓度差别最大达100倍。

Methods The gene fragment of E2 region was obtained from the sera of patients with HCV infection by random primer reverse transcription and polymerase chain reaction ( PCR ), and then cloned into procaryotic expression system to express and Purify the protein and detect its antigenicity .

方法首先从HCV感染者血清中经随机引物反转录和聚合酶链反应（PCR）获得E2区基因片段，然后克隆入原核表达系统pQE30中进行蛋白表达、纯化，并检测表达蛋白的抗原性。

While a T_7 promoter sequence and a M_ ( 13 ) reverse sequence were added on both sides of anchor - primer and random - primer , then DDRT-PCR system have better identical than before process in amplification specificity .

在锚定引物之前加上了一段T7启动子序列，在随机引物前加上一段M13重复序列，可使DDRT&PCR的体系的扩增特异性更一致。

Random amplified polymorphism DNA ( RAPD ) was applying artifical random primer to amplify the gene team being studied by PCR and get polymorphic RAPD fragment , it may detect several gene sites , but could not distinguish heterozygote site ;

RAPD是以人工合成的碱基顺序随机排列的寡核苷酸单链为引物，对所研究的基因组DNA进行PCR扩增，产生多态性的RAPD片段，可以检测多个基因位点，但不能识别杂合子位点；